Jul 28, 2023
Human urine procurement and processing
- hannah.anvari1,
- asma.giornazi1,
- shriya.shah1,
- maryellen.pavone1,
- francesca.e.duncan duncan1
- 1Northwestern Medicine OBGYN, Northwestern University
Protocol Citation: hannah.anvari, asma.giornazi, shriya.shah, maryellen.pavone, francesca.e.duncan duncan 2023. Human urine procurement and processing. protocols.io https://dx.doi.org/10.17504/protocols.io.dm6gp3bmjvzp/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: July 28, 2023
Last Modified: July 28, 2023
Protocol Integer ID: 85637
Funders Acknowledgement:
SenNet
Grant ID: U54AG075932
Abstract
Purpose: This protocol is intended for use in the collection and storage of human urine in a research setting. The protocol details the collection, processing, and long-term storage for downstream analysis alongside other tissues or samples of interest (i.e. follicular fluid, ovary).
Guidelines
Researchers will adhere to all safety and training protocols required by the institution (Northwestern Medicine/Northwestern
University) including but not limited to:
- Biosafety Certification
- Bloodborne Pathogens Certification
- Collaborative Institutional TrainingInitiative (CITI program) Certification
Materials
- Parter Medical Products™ Sterile Specimen Cups (Fisher Scientific, 22-150-265 or equivalent)
- Corning 5 mL Internal Threaded Polypropylene Cryogenic Vial, Self-Standing with Round Bottom (Fisher Scientific, 09-761-69 or equivalent)
Safety warnings
Researchers will wear personal protective equipment when working with human specimens, including gloves, masks, and lab coats.
Ethics statement
Human urine procurement and processing will adhere to the approved IRB protocol approved through NU (NU12G09) for collection of biofluids associated with study participants through Northwestern Medicine.
A urine sample is collected in a standard specimen cup during pre-op from a nurse. Once the urine sample has been collected it is placed immediately on ice following collection. A research coordinator will bring the urine sample to the lab on ice (2-4 °C). Urine samples should be transported in a blue, biohazard labeled cooler bag (Fig. 1A, B).
Fig. 1. Urine collection and processing overview. Urine samples are collected and transported to the research coordinator on ice in a blue biohazard labeled cooler bag (A, B). Upon arrival a photo is taken of the urine biospecimen cup (C) the urine sample is then immediately aliquoted into 5ml cryovials (D) and then stored at -80°C until analyzed.
On arrival the urine sample biospecimen cup is photographed and then aliquoted from the specimen cup (Fig. 1C) into 5 mL cryovials (Fig. 1D) and then stored at -80°C until analysis.