On arrival, tissue should be measured for length, width, and thickness (Fig. 2D). Also note the approximate anatomy of cortex (black) to medulla (white) (Fig. 2E). The cortex should be on the outermost edge surrounding the entire tissue piece and will have a glossy, somewhat translucent appearance. The medulla should be the innermost portion if the ovarian tissue slice is lying flat and will likely contain fibrotic looking, white tissue especially in postmenopausal ovaries. Prepare a 60 mm dish with 3-5 mL ORIGIO Handling media. The tissue should be submerged, but not floating in media.