ORIGIO Handling IVF Medium (ORIGIO, #83100060)
Disposable Scalpel, Sterile, No. 10 and No. 22 (Fisher Scientific, 12-460-456)
15 mL conical tubes (Fisher Scientific, 2610L35) for tissue collection.
Gibco™ DPBS, calcium, magnesium (Fisher Scientific, 14-190-250)
60 mm Dish, Non-Treated, Corning™ Falcon™ Bacteriological Petri Dishes with Lid (Fisher Scientific 08-772-12 or equivalents) for processing
Stadie-riggs tissue slicer (discontinued)
Stadie-Riggs tissue slicer/microtome blades (Thomas Scientific 6727C18)
Stadie-Riggs tissue slicer blade handle (Thomas Scientific 6727C25)
Plastic Discs (Fisher Scientific, 1018001)
MEM Alpha (1X) + GlutaMAX (Thermo, 32561037)
Human serum albumin (Cooper Surgical Inc, ART-3003)
Fetuin (Sigma, F3385-25G)
Insulin-Transferrin-Sodium Selenite Supplement (Sigma-Aldrich, I1884-1VL)
Doxorubicin (Fisher Scientific, 22-521-0)
Millicell Cell Culture Insert, 12 mm, hydrophilic PTFE, 0.4 µm (Millipore Sigma, PICM01250)
Falcon® 24-Well Flat-Bottom Plate, Tissue Culture-Treated (Fisher Scientific, 353047)
Invitrogen™ RNase-free Microfuge Tubes (ThermoFisher Scientific, AM12400)