License: This is an open access collection distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this collection and it's working
Created: March 10, 2023
Last Modified: May 31, 2024
Collection Integer ID: 78543
Keywords: ASAPCRN
Funders Acknowledgement:
The U.S. Army Medical Research Materiel Command endorsed by the U.S. Army through the Parkinson’s Research Program Investigator-Initiated Research
Grant ID: W81XWH1810508
Abstract
This is a collection of protocols that details the entire process of conducting a human microbiome study from start to end. It includes (1) consent form, to enroll subjects with permission to store and share data (2) data collection protocols designed to be self-administered by subjects (Environmental & Family History Questionnaire, Gut Microbiome Questionnaire, instructions for saliva collection, and instructions for stool collection), (3) codes for bioinformatics and statistical data analysis, (4) workflow at a glance created according to STORM guidelines and (5) final product, the manuscript, which describes the whole process from start to end using metagenomics of Parkinson’s disease implicates the gut microbiome in multiple disease mechanisms.
