Oct 12, 2023

Public workspaceHuman Liver Sample Collection Protocol -- University of Minnesota TMCs

 Forked from UMN SenNet Liver Collection Protocol
DOI
dx.doi.org/10.17504/protocols.io.yxmvm3prnl3p/v1
  • 1University of Minnesota, Minneapolis, MN USA;
  • 2University of Minnesota
Allie Pybas
Open access
DOI: dx.doi.org/10.17504/protocols.io.yxmvm3prnl3p/v1
Protocol Citation: Laura J Niedernhofer, David A Bernlohr, Steve Johnson 2023. Human Liver Sample Collection Protocol -- University of Minnesota TMCs. protocols.io https://dx.doi.org/10.17504/protocols.io.yxmvm3prnl3p/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: October 12, 2023
Last Modified: October 12, 2023
Protocol Integer ID: 89212
Funders Acknowledgement:
NIH
Grant ID: 5U54AG079754-02
NIH
Grant ID: 5U54AG076041-03
Abstract
Collection protocol obtained from the attached BioNet Specimen Procurement Agreement provided by the UMN CTSI Biorepository and Laboratory Services (BLS).

Download Tissue Procurment Agreement - 06.2023.pdfTissue Procurment Agreement - 06.2023.pdf343KB

Preparation
Preparation
Patient Identification: As soon as a patient is scheduled, the research team will email bionet@umn.edu a completed Specimen Procurement Request Form.
Patient Consent: Researcher consents. The original signed consent form will be placed in the patient chart and scanned into Epic. BioNet will verify consent in Epic. If the consent is not scanned in EPIC before the procedure, the researcher will provide BioNet a copy direct (e-mail or hard copy).
Collection Supplies: All supplies will be delivered to the BioNet office at least one day before the procedure and appropriately labeled with researcher name and CTSI Project #.

Collection Kit Components: PBS (Study team) Media: DMEM + antibiotics (Study team) Media tubes (x3 per collection) (Study team) 10% Formalin (Bionet) FFPE cassette x 5 (Bionet) Specimen container (Bionet) OCT compound (Bionet) OCT mold x 4 (Bionet) OCT quick freeze box (Bionet) Liquid nitrogen vapor (Bionet) Cryovials x 3 (Bionet) Collection kit containing 10 ml EDTA blood tubes x 2 (Bionet)
Procurement and Processing of Liver Samples
Procurement and Processing of Liver Samples
  1. 2 liver biopsy cores will be collected by surgeon.
  2. The location of the tissue collection will be recorded by the surgeon.
  3. BLS will be paged by OR for immediate specimen pickup.
Core 1:
  1. Surgeon or OR staff will place core 1 in 10% formalin.
  2. BLS procurement will pick up specimen and request Bionet Research Specimen Order.
  3. BLS will tape a labeled FFPE cassette to the top of the specimen container.
  4. BLS procurement will submit specimen to BLS Histology for FFPE processing. Standard fixation period of 24-72 hours. H&E and trichrome stain requested.
Core 2:
  1. Surgeon or OR staff will place core 2 in specimen container and page BLS.
  2. BLS procurement will pick up specimen immediately and request Bionet Research Specimen Order. Short transit/processing time is critical for this specimen.
  3. Prepare OCT quick freeze box by adding liquid nitrogen. This must be done after the core is collected because the liquid nitrogen vapor will dissipate quickly.
  4. Place core 2 in one OCT mold.
  5. Ensure mold is labeled.
  6. Add OCT compound into mold containing core. Ensure no bubbles surround the tissue.
  7. After OCT sample is frozen, remove and transfer to -BOC freezer storage.
If liver is collected as a wedge:
  1. One wedge will be removed in the OR and placed in PBS.
  2. Split wedge in half and treat as core 1 and core 2, processing according to same instructions.