This protocol is an overview of the protocols in use for the single nuclear RNA sequencing and ATACseq datasets for HubMAP-Lung Tissue Mapping Center, TMC UCSD / TMC URMC. It includes protocols.io links to each of the individual protocols that make up this project workflow, from processing tissue, sectioning, H&E imaging the tissue samples, isolation of nuclei from frozen lung tissue, running of multimodal single cell/nucleus assays.uploading the final data to the HuBMAP HIVE.