Apr 08, 2022
HuBMAP | GE/ University of Washington Cell DIVE™ Modality Overview
- 1GE Research
Protocol Citation: Liz McDonough 2022. HuBMAP | GE/ University of Washington Cell DIVE™ Modality Overview. protocols.io https://dx.doi.org/10.17504/protocols.io.3byl4bwmzvo5/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it’s working
Created: April 07, 2022
Last Modified: April 08, 2022
Protocol Integer ID: 60465
Funders Acknowledgement:
Human Biomolecular Atlas Program (HuBMAP)
Grant ID: 3UH3CA246594-02S1
Abstract
This is an overview of all protocols currently in use for the GE/University of Washington Cell DIVE collaboration for the Human BioMolecular Atlas Program (HuBMAP). It includes links to each of the individual protocols that make up this project workflow.
Characterize antibodies (primary/secondary, direct conjugates, and zenon labelled antibodies) and determine any antigen effects from the Cell DIVE dye inactivation process.
Prepare direct conjugates for study.
Perform Cell DIVE™ multiplexed data acquisition on the final cohort.
Note
Staining is done using the Leica Bond MAX and images are acquired on the Leica Cell DIVE imager.