Apr 17, 2024

Public workspaceHow to request an assisted sorting session for the first time

This protocol is a draft, published without a DOI.
  • 1European Molecular Biology Laboratory (EMBL)
Daniel Gimenes
Open access
Protocol Citation: Daniel Gimenes 2024. How to request an assisted sorting session for the first time. protocols.io https://protocols.io/view/how-to-request-an-assisted-sorting-session-for-the-dbef2jbn
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: March 27, 2024
Last Modified: April 17, 2024
Protocol Integer ID: 97447
Keywords: PPMS, EMBL, Flow Cytometry, Core Facility, Cell sorting
Abstract
Step by step protocol to create a PPMS account and to find the "Preliminary Sorting Discussion" form.
Creating a PPMS account
Creating a PPMS account
If you already have a PPMS account, please proceed to the step 6. If not, access the following link: https://ppms.embl.de/start/
Click on Flow Cytometry Core Facility



Click on "user account creation request".



Next, you will be prompted to enter your credentials.
On the "Username" field, please fill with your username without the "@embl.de" (don't use your full email). After that, just use your regular login password



You will be brought to a small form where you can fill the important information for the service billing and once submitted, the staff will receive an account creation request. Once we accept it, you should receive a confirmation by email. 🙂
Requesting an assisted sorting
Requesting an assisted sorting
Click on Flow Cytometry Core Facility



Login into your account



On the upper tabs, click on "ORDER"



  1. Optional: Attach the order to a project if available
  2. Select a financial account for the order or keep the default one (Note that this service is not going to be charged!)
  3. Add 1 to the "Quantity" box for the Preliminary Cell Sorting Request
  4. Click on "Order".



Once you are done filling all the required information, click on "Submit Form".
  1. Facility staff will receive your order, accept it and will check if everything is OK. If potential questions arises, the staff will send them to the user or will ask to schedule a meeting.
  2. When all the questions are clarified, the staff will give the users rights to book the instrument(s).
To book a instrument, refer to the How to book a sorting slot in PPMS protocol