Dec 04, 2024

Public workspaceHi-C protocol

DOI
dx.doi.org/10.17504/protocols.io.14egn924yl5d/v1
  • 1Sloan Kettering Institute;
  • 2Johns Hopkins University
  • IGVF
Michael Beer
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DOI: dx.doi.org/10.17504/protocols.io.14egn924yl5d/v1
Protocol Citation: Renhe Luo, Michael Beer, Danwei Huangfu 2024. Hi-C protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.14egn924yl5d/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: December 04, 2024
Last Modified: December 04, 2024
Protocol Integer ID: 113703
Funders Acknowledgements:
NHGRI
Grant ID: U01HG012051
Abstract
Hi-C protocol for ESC DE differentiation time course.
Hi-C protocol
Hi-C protocol
2 million cells were collected and fixed with 1% formaldehyde.
The subsequent steps of Hi-C were then performed using the Arima-HiC kit (Arima; A510008) while libraries for sequencing were prepared with the KAPA Hyper Prep Kit (KAPA; KK8502) following the manufacturers’ guidelines.
Samples were pooled and submitted to MSKCC Integrated Genomics Operation core for quality control and sequencing on Illumina HiSeq 4000 platform.