Feb 06, 2023

Public workspaceHEPES transformation buffer

This protocol is a draft, published without a DOI.
  • 1University of Oslo
Andreas Sagen
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Protocol Citation: Andreas Sagen 2023. HEPES transformation buffer. protocols.io https://protocols.io/view/hepes-transformation-buffer-cnvcve2w
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: February 05, 2023
Last Modified: February 06, 2023
Protocol Integer ID: 76420
Keywords: transformation buffer, HEPES buffer, electroporation, mechanical transformation
Abstract
HEPES buffer is commonly used to prepare prokaryotic (bacterial) cells for electroporation.
Materials
LAF bench
Scale
Filter unit
Protocol materials
ReagentHEPESMerck MilliporeSigma (Sigma-Aldrich)Catalog #H4034
Step 2
1 M HEPES buffer
1 M HEPES buffer
Add Amount40 mL distilled water to a Amount50 mL tube
Measure and add Amount11.915 g HEPES Materials: ReagentHEPESSigma AldrichCatalog #H4034
Adjust pH to Ph7.4 with concentrated sodium hydroxide or hydrogen chloride
Add distilled water to Amount50 mL
Filter sterilize with a 0.2 µm pore-size filter