Oct 08, 2024
Version 2
H_QTof_PL_v2 V.2
- Fang Bian1
- 1University of Texas at Dallas
Protocol Citation: Fang Bian 2024. H_QTof_PL_v2. protocols.io https://dx.doi.org/10.17504/protocols.io.n92ld8xr8v5b/v2Version created by Mass Spec Core
Manuscript citation:
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License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: September 18, 2024
Last Modified: October 08, 2024
Protocol Integer ID: 107928
Keywords: plasma, lipids, extraction
Funders Acknowledgement:
NSF
Grant ID: 2018188
Abstract
To extract plasma lipids for LCMS analysis.
Guidelines
Use glassware throughout this protocol.
Materials
Fishers 3 ml glass tube
Glass tube cap
Glass pipette tips
Regular pipette tips for plasma only.
Before start
Use LCMS grade water and solvents.
To extract plasma lipids for LCMS study
To extract plasma lipids for LCMS study
Transfer plasma 50 ul (regular pipette tips) into a 3 ml glass tube
Add 200 ul of LCMS grade-chloroform/methanol (2:1)
Stand for 5 min at room temperature.
Vortex for 1 min
Centrifuge 12,000 g, for 5 min at 4c
Use glass pipette tips to collect the lower organic phase and transfer the liquid to a new glass tube.
Repeat steps 2-5
Use glass pipette tips to collect the lower organic phase and transfer the same tube in step 6.
Evaporate the extracted solvents to dryness
Cap the tube, store at -20oC
Deliver the sample to mass spec core.