Apr 03, 2023

Public workspaceGlucocerebrosidase is imported into mitochondria and preserves complex I integrity and energy metabolism - ASAP protocol collection

  • 1German Center for Neurodegenerative Diseases (DZNE), Tübingen, 72076 Germany
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Collection Citationmichela.deleidi, Pascale Baden, María José Pérez J., Hariam Raji, Federico Bertoli 2023. Glucocerebrosidase is imported into mitochondria and preserves complex I integrity and energy metabolism - ASAP protocol collection. protocols.io https://dx.doi.org/10.17504/protocols.io.x54v9d44mg3e/v1
License: This is an open access collection distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this collection and it's working
Created: April 03, 2023
Last Modified: May 31, 2024
Collection Integer ID: 79922
Keywords: ASAPCRN, Glucocerebrosidase is imported into mitochondria and preserves complex I integrity and energy metabolism
Funders Acknowledgement:
Aligning Science Across Parkinson’s through the Michael J. Fox Foundation for Parkinson’s Research (MJFF)
Grant ID: ASAP-000420
EU Joint Program—Neurodegenerative Disease Research (JPND) project
Grant ID: GBA-PaCTS, 01ED2005B
Abstract
Collection of protocols of Deleidi Lab used in the publication: "Glucocerebrosidase is imported into mitochondria and preserves complex I integrity and energy metabolism"
Files
Protocol
Icon representing the file Gcase co-immunoprecipitation
Name
Gcase co-immunoprecipitation
Version 1
, ASAP
Federico BertoliASAP
Protocol
Icon representing the file Single cell dissociation of brain organoids
Name
Single cell dissociation of brain organoids
Version 1
, ASAP
Federico BertoliASAP
Protocol
Icon representing the file Plasmid-reprogramming of human fibroblasts
Name
Plasmid-reprogramming of human fibroblasts
Version 1
, ASAP
Federico BertoliASAP
Protocol
Icon representing the file Sequencing of construct
Name
Sequencing of construct
Version 1
, ASAP
Federico BertoliASAP
Protocol
Icon representing the file Single cell analysis of iPSC-derived midbrain organoids
Name
Single cell analysis of iPSC-derived midbrain organoids
Version 1
, ASAP
Federico BertoliASAP
Protocol
Icon representing the file Complex I activity assay
Name
Complex I activity assay
Version 1
, ASAP
Federico BertoliASAP
Protocol
Icon representing the file Midbrain-like Organoids generation from hiPSCs
Name
Midbrain-like Organoids generation from hiPSCs
Version 1
, ASAP
Federico BertoliASAP
Protocol
Icon representing the file Endogenous coimmunoprecipitation
Name
Endogenous coimmunoprecipitation
Version 1
, ASAP
Federico BertoliASAP
Protocol
Icon representing the file Generation of induced pluripotent stem cells and gene correction
Name
Generation of induced pluripotent stem cells and gene correction
Version 1
, ASAP
Federico BertoliASAP
Protocol
Icon representing the file Virus production
Name
Virus production
Version 1
, ASAP
Federico BertoliASAP
Protocol
Icon representing the file Live-cell imaging
Name
Live-cell imaging
Version 1
,
Hariam Raji
Protocol
Icon representing the file Expansion microscopy
Name
Expansion microscopy
Version 1
, ASAP
Federico BertoliASAP
Protocol
Icon representing the file Western Blot Analysis
Name
Western Blot Analysis
Version 1
, ASAP
Federico BertoliASAP
Protocol
Icon representing the file Soluble and insoluble A-SYN fractionation
Name
Soluble and insoluble A-SYN fractionation
Version 1
, ASAP
Federico BertoliASAP
Protocol
Icon representing the file Mitochondrial complex activity assays
Name
Mitochondrial complex activity assays
Version 1
, ASAP
Federico BertoliASAP
Protocol
Icon representing the file Neuromelanin staining (Fontana-Masson staining)+ TH-DAB staining on midbrain organoids
Name
Neuromelanin staining (Fontana-Masson staining)+ TH-DAB staining on midbrain organoids
Version 1
, ASAP
Federico BertoliASAP
Protocol
Icon representing the file Differentiation	NPCs	to Dopaminergic/Midbrain Neurons
Name
Differentiation NPCs to Dopaminergic/Midbrain Neurons
Version 1
, ASAP
Federico BertoliASAP
Protocol
Icon representing the file Immunofluorescence staining
Name
Immunofluorescence staining
Version 1
,
Hariam Raji
Protocol
Icon representing the file In vitro GCase activity assay (total cell lysate)
Name
In vitro GCase activity assay (total cell lysate)
Version 1
, ASAP
Federico BertoliASAP
Protocol
Icon representing the file Flag co-immunoprecipitation
Name
Flag co-immunoprecipitation
Version 1
, ASAP
Federico BertoliASAP
Protocol
Icon representing the file Constructs and generation of stable cell lines
Name
Constructs and generation of stable cell lines
Version 1
, ASAP
Federico BertoliASAP