Protocol Citation: Sabina Marciano, Roberta Marongiu 2023. GI Transit Assay. protocols.io https://dx.doi.org/10.17504/protocols.io.eq2lyjj3mlx9/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: August 14, 2023
Last Modified: May 31, 2024
Protocol Integer ID: 86471
Keywords: ASAPCRN
Abstract
This test is used to check for possible signs of constipation and alteration in the gut transit
Materials
Carmine red (Sigma-Aldrich, St. Louis MO)
Fast mice for 6 hours
Administer 0.3 mL Carmine red per mouse via oral gavage
Monitor the mice every 15 minutes for up to 5 hours for the appearance of the first red fecal pellet
The time from gavage to passage of first red pellet is recorded as total intestinal transit time.