A | B | C | |
Equipment | Source | Part Numbers | |
Baking oven | Quincy Lab, Inc. (or comparable) Boekel Scientific ACDBio | Various GC models | |
Hybridization oven including hybridization chamber *: Boekel Scientific RapidFISH Slide Hybridizer or HybEZ II Hybridization System l HybEZ oven l Humidity control tray l EZ-Batch Wash Tray l EZ-Batch Slide Holder l HybEZ Humidifying Paper (2 sheets) | Boekel Scientific ACDBio | 240200 for 120V or 240200-2 for 230V 321710/321720 310012 321717 321716 310025 | |
Water bath (up to at least 37°C) | Various | Various | |
UV Light Box (optional) | VWR | Various | |
5-quart Steamer | Nesco or Hamilton Beach Note: Products from other vendors may require testing and optimization. | ST-25F 37530Z |
A | B | C | |
Materials | Source | Part Numbers | |
Pipettes for 5–1,000 μL | Various | Various | |
12-channel P20 multi-channel pipette | Various | Various | |
Filter Tips (RNase/DNase free) | Various | Various | |
Microtubes | Sarstedt (or comparable) | 72.785.005 | |
Superfrost Plus microscope slides | Fisher Scientific (or comparable) | 12-550-15 | |
Tissue Tek Slide Stain Dish (plastic Coplin Jars) or equivalent (recommend ~16) | Sakura (or comparable) | 25608-904 or 25608-906 | |
Instant-read digital thermometer | Various | Various | |
Humidity Chamber | Simport | M920-1 | |
Benchtop protector sheet (fits inside the hybridization oven, optional) | Fisher Scientific (or comparable) | 15235101 | |
HybriSlip hybridization covers (22 mm x 40 mm x 0.25 mm) Note: this has been validated by NanoString. Do not use other products | Grace Bio-Labs | 714022 | |
RNase AWAY | ThermoFisher | 7003PK | |
Heat/cold protectant handling glove | Various | Various | |
Forceps (for slide handling) | Various | Various | |
Aluminum foil | Various | Various |
A | B | C | |
Reagent | Dilution | Storage | |
95% EtOH | Prepare 500 mL of 95% ethanol by adding 25 mL of DEPC- treated water to 475 mL of 100% ethanol. Change at least weekly. | RT | |
1X PBS pH 7.4 | Prepare 1 L of 1X PBS by combining 100 mL of 10X PBS and 900 mL of DEPC-treated water. Don't reuse. | 4°C | |
10% neutral buffered formalin (NBF) | Caution: NBF is hazardous, handle with care and minimize inhalation risks. Work with 10% NBF in the fume hood. OK to reuse. | RT | |
Proteinase K | Prepare 1 µg/mL by adding 10 μL of 20 mg/mL Proteinase K to 200 mL of 1X PBS made with DEPC-treated water Note: Should be prepared fresh daily; don't reuse. Take care to pipette accurately. Inaccurate concentration of Proteinase K will affect assay performance. Serial dilutions are recommended. | RT | |
NBF stop buffer | Prepare 24.5 g Tris base and 15 g Glycine to 2 L DEPC- treated water. Do not reuse. Solution will lose efficacy with repeated use. | RT | |
2X SSC | Prepare 1 L of 2X SSC by combining 100 mL of 20X SSC and 900 mL of DEPC-treated water. Do not reuse. | RT | |
2X SSC-T | Prepare 100 mL of 2X SSC by combining 10 mL of 20X SSC, 1 mL of 10% Tween-20, and 89 mL of DEPC-treated water. Do not reuse. | RT | |
4X SSC | Prepare 1 L of 4X SSC by combining 200 mL of 20X SSC and 800 mL of DEPC-treated water. Do not reuse. | RT |