Jul 16, 2023
Gastrointestinal transit
- 1Columbia University
Protocol Citation: Connor Monahan 2023. Gastrointestinal transit. protocols.io https://dx.doi.org/10.17504/protocols.io.4r3l22464l1y/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: July 12, 2023
Last Modified: May 31, 2024
Protocol Integer ID: 84891
Keywords: Gastrointestinal transit, constipation, carmine red, motility, ASAPCRN
Funders Acknowledgement:
Aligning Science Across Parkinson's
Grant ID: 0375
Abstract
This protocol details measuring gastrointestinal transit time in mice
Attachments
786-1996.pdf
39KB
Materials
Reagents
CarmineMerck MilliporeSigma (Sigma-Aldrich)Catalog #C1022
0.5% methylcelluloseMerck MilliporeSigma (Sigma-Aldrich)Catalog #M0512
Procedure
Procedure
1h 10m
1h 10m
Prepare a sterile solution of carmine red (300 µl; 6%; Sigma-Aldrich, Cat #C1022; St Louis, MO) suspended in 0.5% methylcellulose (Sigma-Aldrich, Cat #M0512; St Louis, MO).
Administer 0.3 mL of carmine red solution by gavage through a 21-gauge round-tip feeding
needle.
The time at which gavage takes place is recorded as T0.
Place each mouse in separate cages without bedding.
01:00:00 after gavage, monitor fecal pellets at 00:10:00 intervals for the presence of carmine red. Total GI transit time is considered as the interval between T0 and the time of first observance of carmine red in the stool.
1h 10m