Feb 04, 2025

Public workspaceβ-Galactosidase staining

DOI
dx.doi.org/10.17504/protocols.io.14egn9zdzl5d/v1
  • 1Institut Imagine
  • Team Deleidi
Federico Bertoli
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DOI: dx.doi.org/10.17504/protocols.io.14egn9zdzl5d/v1
Protocol Citation: Maria Jose Perez J. 2025. β-Galactosidase staining. protocols.io https://dx.doi.org/10.17504/protocols.io.14egn9zdzl5d/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: November 27, 2024
Last Modified: February 04, 2025
Protocol Integer ID: 112827
Funders Acknowledgements:
ASAP
Abstract
β-Galactosidase staining
For cell staining
For cell staining
Culture cells on Matrigel-coated coverslips.
Fix cells with the fixation solution provided in the Senescence β-Galactosidase Staining Kit (Cat. number 9860; Cell Signaling Technology).
For tissue and organoid sections
For tissue and organoid sections
Prepare 20 µm OCT-embedded sections by cryosectioning.
Mount sections on Epredia‱ SuperFrost‱ Plus slides (Fisher Scientific).
Refix sections with the fixation solution provided in the staining kit.
After fixation for both cells and sections
After fixation for both cells and sections
Perform β-Gal staining on both cells and sections according to the manufacturer’s instructions.
Acquire images:
  • Use a Leica DM750 brightfield microscope with a 40×/0.4 numerical aperture objective for cells.
  • Use an EVOS M7000 Imaging System with a 20×/0.4 numerical aperture for tissue and organoid sections.