Oct 04, 2024

Public workspaceFUNDIS DNA Extraction, PCR, Oxford Nanopore Technologies MinION Sequencing and data analysis.  V.1

DOI
dx.doi.org/10.17504/protocols.io.36wgqnweygk5/v1
  • 1FUNDIS, Dikarya LLC, NAMA, California State University, East Bay;
  • 2Biodiverse
Harte Singer
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DOI: dx.doi.org/10.17504/protocols.io.36wgqnweygk5/v1
Collection Citation: Harte Singer, Stephen Douglas Russell 2024. FUNDIS DNA Extraction, PCR, Oxford Nanopore Technologies MinION Sequencing and data analysis. . protocols.io https://dx.doi.org/10.17504/protocols.io.36wgqnweygk5/v1
License: This is an open access collection distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: In development
We are still developing and optimizing this collection
Created: October 04, 2024
Last Modified: October 04, 2024
Collection Integer ID: 109190
Keywords: DNA Barcoding, Fungi, Mycology, Nanopore, nrITS, Oxford Nanopore Technologies, MinION, PCR
Abstract
Generating DNA barcode sequences from fungal samples using the Oxford Nanopore Technologies MinION platform. Some methods adapted from Stephen D. Russell's protocols dx.doi.org/10.17504/protocols.io.36wgq7qykvk5/v3
Files
Protocol
Icon representing the file FUNDIS ONT Post-PCR Pooling & Purification for Fungal Barcoding
Name
ForkFUNDIS ONT Post-PCR Pooling & Purification for Fungal Barcoding
Version 1
, FUNDIS
Harte SingerFUNDIS
Protocol
Icon representing the file FUNDIS DNA Extraction
Name
FUNDIS DNA Extraction
Version 2
, FUNDIS
Harte SingerFUNDIS
Protocol
Icon representing the file FUNDIS Setting up PCR for rDNA ITS Barcoding of Fungi using ONT Nanopore
Name
FUNDIS Setting up PCR for rDNA ITS Barcoding of Fungi using ONT Nanopore
Version 2
, FUNDIS
Harte SingerFUNDIS
Protocol
Icon representing the file DNAMA VSVC Guidebook
Name
DNAMA VSVC Guidebook
Version 1
, FUNDIS
Harte SingerFUNDIS
Protocol
Icon representing the file FUNDIS ONT V14 Nanopore Adapter Ligation for Fungal DNA Barcoding Flongle 10.4.1
Name
ForkFUNDIS ONT V14 Nanopore Adapter Ligation for Fungal DNA Barcoding Flongle 10.4.1
Version 1
, FUNDIS
Harte SingerFUNDIS
Protocol
Icon representing the file FUNDIS version ONT dA-tailing for Fungal Barcoding
Name
ForkFUNDIS version ONT dA-tailing for Fungal Barcoding
Version 1
, FUNDIS
Harte SingerFUNDIS
Protocol
Icon representing the file FUNDIS Fungal Tissue Sampling for PCR
Name
FUNDIS Fungal Tissue Sampling for PCR
Version 1
, FUNDIS
Harte SingerFUNDIS