Apr 11, 2022

Public workspaceFunctional Magnetic Resonance Imaging Under Swallowing Task in Healthy Human Subjects and Gastroparetic Patients

DOI
dx.doi.org/10.17504/protocols.io.x54v9j4d1g3e/v1
  • Kun-Han Lu1,
  • Kristine Mosier2,
  • John Wo2,
  • Terry Powley1
  • 1Purdue University;
  • 2Indiana University School of Medicine
Kun-Han Lu
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DOI: dx.doi.org/10.17504/protocols.io.x54v9j4d1g3e/v1
Protocol Citation: Kun-Han Lu, Kristine Mosier, John Wo, Terry Powley 2022. Functional Magnetic Resonance Imaging Under Swallowing Task in Healthy Human Subjects and Gastroparetic Patients. protocols.io https://dx.doi.org/10.17504/protocols.io.x54v9j4d1g3e/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it’s working
Created: July 29, 2021
Last Modified: April 11, 2022
Protocol Integer ID: 51970
Keywords: fMRI, swallowing, human, gastroparesis
Funders Acknowledgement:
NIH SPARC
Grant ID: OT2OD023847
Abstract
This protocol described the steps to acquire and analyze functional magnetic resonance imaging data under a swallowing task in healthy human subjects and patients with gastroparesis.
Subjects
Subjects
Eight healthy subjects (6 females; 2 males) and 3 gastroparetic patients (2 females; 1 male) participated in this study under research protocols approved by the Institutional Review Board at Indiana University School of Medicine. All healthy subjects did not have a prior diagnosis of neurological, psychiatric, or gastrointestinal (GI) disorders. All gastroparetic patients had documented delayed gastric emptying by a standardized 4-h gastric emptying scintigraphy test (Tougas et al., 2000). Written informed consent was obtained from all participants. Standard MRI exclusion criteria were applied.
Experiment design
Experiment design
Every subject underwent 3 fMRI sessions. Each subject first had a 5-min, eyes-closed, resting-state fMRI scan. Then, the subject was instructed to perform a 30-sec ON, 30-sec OFF swallowing task during a 5-min fMRI scan. The subject was asked to continuously swallow boluses of water during each 30-sec ON period via a silastic tube and stop swallowing during the 30-sec OFF period. After the swallowing fMRI scan, the subject had another 5-min, eyes-closed, resting-state fMRI scan.
MRI acquisition
MRI acquisition
T1 and T2-weighted MRI and fMRI data were acquired in a 3T MRI system (Siemens, Magnetom Prisma, Germany) with a 64-channel receive-only phased-array head/neck coil. The fMRI data were acquired with 2 mm isotropic spatial resolution and 1s temporal resolution by using a gradient-recalled echo-planar imaging sequence (multiband = 8, 72 interleaved axial slices, TR = 1000 ms, TE = 31 ms, flip angle = 52°, field-of-view = 21 cm × 21 cm). MRI/fMRI images were preprocessed using a similar pipeline as in the Human Connectome Project (Glasser et al., 2013). Briefly, all fMRI images were corrected for motion and slice timing, aligned to structural images, and normalized to the Montreal Neurological Institute (MNI) space.