Nov 02, 2023
- 1University of Cambridge
Protocol Citation: Alexander Röntgen 2023. FTIR. protocols.io https://dx.doi.org/10.17504/protocols.io.dm6gp3xqpvzp/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: October 31, 2023
Last Modified: November 02, 2023
Protocol Integer ID: 90237
Abstract
Protein FTIR protocol.
Materials
Vertex 70 FTIR spectrometer with a DiamondATR unit and a deuterated lanthanum α‑alanine-doped triglycine sulphate detector (Bruker)
Background scan
Background scan
- Spot 5 µL of buffer on prism
- Acquire background scan
Sample scan
Sample scan
- Spot 5 µL of protein solution on prism
- Acquire sample scan