Mar 08, 2025
Version 3
Fragment Analyzer Operation for PCR Products V.3
- Allen Institute for Brain Science1
- 1Allen Institute
- Allen Institute for Brain ScienceTech. support
Protocol Citation: Allen Institute for Brain Science 2025. Fragment Analyzer Operation for PCR Products. protocols.io https://dx.doi.org/10.17504/protocols.io.5jyl8mxwrg2w/v3Version created by Allen Institute
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: June 03, 2020
Last Modified: March 08, 2025
Protocol Integer ID: 124014
Keywords: MB0073, quality control, QC, genotyping, polymerase chain reaction, products,
Abstract
This protocol describes fragment analyzer operation for polymerase chain reaction (PCR) products. The Fragment Analyzer by Advanced Analytical is used as a quality control step for genotyping PCR products. Peak size, shape, and sample concentration are recorded and used to evaluate samples on a pass/fail level.
Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
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