Two 5-minute washes in excess RNA Scope Wash Buffer (ACD) took place between each incubation in sequential order: probes (2-hours), AMP1 (30 min), AMP2 (30 min), AMP3 (15 min), HRP-C1/2/3 (15 min), TSA Cy3 (30 min), HRP blocker (30 min), HRP-C1/2/3 (15 min), and TSA Cy5 (30 min).