Cytometer Voltage/Gain and threshold settings are subjective due to their dependency on alignment, and the scatter filters in place, amongst other variables. The following are guide values to start with and may need adjustment for optimal acquisition.
Beckman Coulter, CytoFLEX [405 nm OD0 filter, 488 nm OD2 filter]
Cytek Bioscience, Aurora [405 nm OD0 filter, 488 nm OD2 filter]