N. benthamiana plants (5 weeks old, grown under long day conditions [16h/8h] at 25°C/22°C and 65% humidity)
Agrobacterium tumefaciens GV3101 harboring pSoup (Hellens et al., 2000)
mCitrine and mScarlet-I constructs (Petutschnig et al.):
pGreenII-0229-p35S-mCitrine
pGreenII-0229-p35S-mScarlet-I
pGreenII-0229-p35S-mCitrine-mScarlet-I
10g/l peptone (Gibco 211677)
5g/l yeast extract (Gibco 212750)
10g/l NaCl2 (Carl Roth 3957)
for plates 15g/l agar (BD Difco, 214530)
Rifampicin, (Duchefa Biochemie R0146), final concentration 20µg/ml
Gentamycin, (Duchefa Biochemie G0124), final concentration 50µg/ml
Kanamycin, (Carl Roth T832), final concentration 50µg/ml
Tetracycline, (Carl Roth 0237), final concentration 5µg/ml
Infiltration medium:
10mM MgCl2 (Carl Roth 2189)
10mM MES pH5,4 (Carl Roth 4256)
150µM acetosyringone (SigmaAldrich D134406)
5 ml Luer-Lock syringe (MediWare I3 0502)
Luer-Lock Combi-Stopper syringe caps (B.Braun 4495101)
Leica Microsystems TCS SP8 Falcon system equipped with HyD SMD detectors, a pulsed white light laser and a HC PL APO 40x/1.10 W CORR CS2 objective or similar.