Primary antibodies for Western blots:
for mCitrine: rat monoclonal anti-GFP (Chromotek, 3H9),
for mScarlet-I: rat monoclonal anti-RFP (Chromotek, 5F8)
Secondary antibodies for Western blots:
Goat-Anti-Rat AP-conjugate (Sigma-Aldrich, A8438)
Goat-Anti-Rabbit AP-conjugate (Sigma-Aldrich, A3687)
use Pierce Western Blot Signal Enhancer (Thermo Fisher Scientific, 21050)
Detection substrate:
Immunstar-AP (Bio-Rad, 1705018)
Detection system:
Bio-Rad Chemidoc
High purity chitin (Sigma Aldrich /Merck, C9752).
Rough-surfaced glass pistils that fit 1.5ml microcentrifuge tubes (Glasgeraetebau Ochs, Bovenden, Germany).
IKA RW 20 overhead stirrer
Leica Microsystems TCS SP8 Falcon system equipped with HyD SMD detectors, a pulsed white light laser and a HC PL APO 40x/1.10 W CORR CS2 objective or similar.