A) CD8+T Cells previously gated on lymphocytes (FSC vs SSC) and 7-AAD negative events, were stained with FITC anti-CD8a and an exclusion cocktail containing Alexa Fluor® 700 anti-CD4, CD19, CD14, and CD16.
B) Antigen specific CD8+T Cells, gated as described, were detected with Flex-T™ tagged with PE and APC. HLA-A*11:01 Flex-T™ was loaded with an EBV peptide (IVTDFSVIK).