Dec 08, 2023
Fixation and embedding of organoids for histology
- 1Children's Hospital of Philadelphia, Gastrointestinal Epithelium Modeling Program
Protocol Citation: Tatiana Karakasheva 2023. Fixation and embedding of organoids for histology. protocols.io https://dx.doi.org/10.17504/protocols.io.5qpvo3w6xv4o/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: December 08, 2023
Last Modified: December 12, 2023
Protocol Integer ID: 92040
Funders Acknowledgement:
Children's Hospital of Philadelphia Research Institute
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Abstract
This is a modification of a previously published protocol for fixation and pre-embedding of 3D organoids prior to embedding into paraffin and sectioning for histology (see Reference). This new protocol is simplified, yet results in the same quality of FFPE sections.
Protocol references
Karakasheva TA, Kijima T, Shimonosono M, Maekawa H, Sahu V, Gabre JT, Cruz-Acuña R, Giroux V, Sangwan V, Whelan KA, Natsugoe S, Yoon AJ, Philipone E, Klein-Szanto AJ, Ginsberg GG, Falk GW, Abrams JA, Que J, Basu D, Ferri L, Diehl JA, Bass AJ, Wang TC, Rustgi AK, Nakagawa H. Generation and Characterization of Patient-Derived Head and Neck, Oral, and Esophageal Cancer Organoids. Curr Protoc Stem Cell Biol. 2020 Jun;53(1):e109. doi: 10.1002/cpsc.109. PMID: 32294323; PMCID: PMC7350550.