Jun 21, 2023
Version 1
FIVTools Overview V.1
- 1Washington University in St. Louis
Protocol Citation: William J Buchser 2023. FIVTools Overview. protocols.io https://dx.doi.org/10.17504/protocols.io.rm7vzxn62gx1/v1
Manuscript citation:
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: In development
We are still developing and optimizing this protocol
Created: June 20, 2023
Last Modified: June 22, 2023
Protocol Integer ID: 83750
Funders Acknowledgement:
MGI
Abstract
Starting point for using FIVTools for image-based functional tools
Installation
Installation
FIVTools is software designed for use with image-based screens and tools from Washington University's FIVE@MGI and collaborators.
Software
FIVTools
NAME
Windows 10+
OS
William Buchser
DEVELOPER
SOURCE LINK
Within WashU, the program is accessed from the RIS archive below. Alternatively, this open-source program can be downloaded from the gitlab repo and run directly.
Loading Image Folder List
Loading Image Folder List
Open the program. (It may ask to install an update which you can).
Below, you can choose whether the images come from the InCell or ImageXPress microscopes (select the InCell path), or if they are "Non-Standard" (anything else).
Images from a Non-Standard Source2 steps
Click "Open Any Folder"
Select an image, then press OK.
Tell FIVTools some of the information about these images
The functionality of this is currently limited, images from InCell or ImageXpress are the primary way to use this software