A | B | |
RNA solution 1ng-1 µg total RNA or 1-100 ng polyA-selected RNA Primer (50 µM dT or 60 µM Random Primer Mix) | 2 µl | |
10 mM dNTP mix | 1 µl | |
Nuclease-free H2O | to final volume of 12 µl |
A | B | |
5X ProtoScript II RT Reaction buffer | 4 µl | |
0.1 M DTT | 2 µl | |
Murine RNase inhibitor (40 U/µl) | 1 µl | |
ProtoScript II Reverse Transcriptase (200 U/µl) | 1 µl | |
Final volume | 20 µl |