Protocol Citation: Sabina Marciano, Roberta Marongiu 2023. Fecal Water Content Assay. protocols.io https://dx.doi.org/10.17504/protocols.io.kqdg3xx5zg25/v1
Manuscript citation:
https://pubs.rsc.org/en/content/articlelanding/2017/FO/C6FO01559J
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: August 14, 2023
Last Modified: May 31, 2024
Protocol Integer ID: 86468
Keywords: ASAPCRN
Abstract
This test is used to check for signs of constipation
Label, weight and record the weight of each Eppendorf tube
Collect 2 pellets from each mouse and weigh.
Dry the pellets in the oven at 60 degrees C for 24 hrs
Weigh the pellets again after drying
Calculate fecal water content according to the equation:
(fecal weight before drying − fecal weight after drying)/feces weight before drying × 100.
Protocol references
https://pubs.rsc.org/en/content/articlelanding/2017/FO/C6FO01559J