Jul 12, 2024
Fecal sample collection
- daniel.dautan daniel1,2,
- Per Svenningsson1,2
- 1Department of Clinical Neuroscience, Karolinska Institutet, 171 76 Stockholm, Sweden;
- 2Aligning Science Across Parkinson’s (ASAP) Collaborative Research Network, Chevy Chase, MD 20815, USA
Protocol Citation: daniel.dautan daniel, Per Svenningsson 2024. Fecal sample collection. protocols.io https://dx.doi.org/10.17504/protocols.io.3byl4qp22vo5/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: February 22, 2024
Last Modified: July 12, 2024
Protocol Integer ID: 95641
Keywords: ASAPCRN, mouse, gut microbiome, fecal
Funders Acknowledgement:
Aligning Science Across Parkinson's
Grant ID: 020608
Abstract
Fecal sample collection from mice to use for gut microbiome sequencing analysis.
Set up the experiment by arranging clean cages without food, water or bedding. It is advisable to label each cage to avoid confusion later on.
In front of each cage, place a glass filled with dry ice and a 1.5ml Eppendorf tube to
speed up collection. Use plastic forceps each cage. Avoid using the same forceps to avoid contamination between samples.
Place the mice in their respective cages.
Collect all fecal pellets produced, and place it on the tube on dry ice. Do not collect pellets that were in urine or dried up pellets.
Transfer quickly the tube into a -80 degree fridge until DNA extraction.