Mar 01, 2022
Fear Conditioning
- 1University of Ottawa
Protocol Citation: Haley Geertsma 2022. Fear Conditioning. protocols.io https://dx.doi.org/10.17504/protocols.io.b5suq6ew
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it’s working
Created: March 01, 2022
Last Modified: March 01, 2022
Protocol Integer ID: 58932
Abstract
This protocol is used to test mice in the Fear Conditioning behavioural test.
Materials
Fear Conditioning apparatus
On day 1, confirm that EthoVision is set up properly for a 6-minute test (with 3 tone-shock pairings of a 30-second tone co-terminated with a 2-second foot shock).
1m
Place mice into the Fear Conditioning apparatus and start the testing.
6m
Once testing is complete, return mice to their home cage overnight.
1d
On day 2, confirm that EthoVision is set up properly for a 6-minute test (no tone or foot shock).
1m
Place mice into the Fear Conditioning apparatus and start the testing.
6m
Once testing is complete, return mice to their home cage overnight.
1d
On day 3, confirm that EthoVision is set up properly for a 6-minute test (with 3 minutes of no tone/shock, then 3 minutes repeating the 30-second tone without a foot shock). Confirm that the room has a novel scent, noise, and Fear Conditioning apparatus).
1m
Place mice into the Fear Conditioning apparatus and start the testing.
6m
Once testing is complete, return mice to their home cage.
1m