Purified water (i.e. distilled or deionized)
Water baths or heating blocks
Sterile glass Erlenmeyer flasks
Electrophoresis power supply
Fungal growth medium appropriate for the species
2- mercaptoethanol (stock bottle from supplier)
1 M dithiothreitol (DTT, Cleland's reagent)
Zymolyase (yeast lytic enzyme)
10% sodium dodecyl sulfate (SDS; filter sterilize, do not autoclave)
Buffer-saturated phenol for molecular biology
Chloroform for molecular biology
10 mM Tris-HCl/1 mM EDTA pH 8.0 (1x TE buffer)
RNase for molecular biology
Proteinase K for molecular biology
Agarose gel for electrophoresis
Electrophoresis gel buffer