Wizard® Enviro Total Nucleic Acid Kit, Promega cat# A2991
Contains sufficient materials and reagents for 25 samples. Includes:
• 1 × 13ml Protease Solution
• 1 × 320ml Binding Buffer 1 (BBD)
• 1 × 30ml Binding Buffer 2 (BBE)
• 1 × 85.3ml Column Wash 1(CWE)
• 1 × 206ml Column Wash 2 (RWA)
• 1 × 150ml Nuclease-Free Water
• 5 × 5 each PureYield™ Binding Columns
• 1 × 25 each PureYield™ Minicolumns
• 1 × 25 each PureYield™ Collection Tubes
• 1 × 50 each Elution Tubes
• 1 × 25 each Reservoir Extension Funnel
Storage Conditions: Store all components at +15°C to +30°C.
Materials to Be Supplied by the User
• tabletop centrifuge (capable of 3,000 × g)
• swinging bucket rotor (that accommodates 50ml tubes)
• 50ml disposable plastic screw-cap tubes (e.g., Corning® or Falcon® brand)
• 1.5ml microcentrifuge tubes
• heat block (capable of reaching 60°C)
• high-speed microcentrifuge (for tubes, capable of at least 10,000rpm)
• vacuum manifold (e.g., Vac-Man® Laboratory Vacuum Manifold, Cat.# A7231)
• Eluator™ Vacuum Elution Device (Cat.# A1071)
• vacuum pump, single- or double-stage, producing pressure of approximately 650mm Hg (25.6 inches Hg,