**In preliminary trials, ethyl acetate and water were used to attempt to extract the compounds, however, the liquids were not as colored suggesting that the solvent was not optimal for extracting the compounds. Therefore, ethanol was used to dissolve the solid in the vials and within 2 minutes the liquid showed a color change.
**Also, in preliminary studies, 100 mg of each solid was used to dissolve the vegetables (i.e. spinach, Red cabbage (but its color is purple), tomatoes, and carrots) in 1 mL of ethanol. However, the spots on the TLC plates after CAM stain did not appear very clearly (they were too faint). Therefore, 500 mg of solid was weighed out and the spots appeared on the TLC plate.
**Solvent system for the TLC chamber was used as 1:4, ethyl acetate to cyclohexane, v/v, since in the preliminary trial, when 1:1, ethyl acetate to cyclohexane, v/v, was used as the solvent system, there were dark spots at the solvent front. The spots at the solvent front in 1:1 EA/cyclohexane suggested that the solvent system was too polar. Therefore, the solvent system of 1:4 EA/cyclohexane was used in addition to the 1:1 EA/cyclohexane solvent system.
**When 1 mL of ethanol was added to 500 mg of fish flakes, the liquid was all soaked up by the solid. Therefore, a total of 3 mL of ethanol was added to the vial containing the fish flakes.
***In the video, the red cabbage was spotted first then spinach. The order of the rest of the spots followed how the items were weighed in Step A.