Oct 14, 2024
EXTRACTION OF DNA BY PHENOL CHLOROFORM METHOD.
- Behjat Ul Mudassir1
- 1COMSATS UNIVERSITY ISLAMABAD PAKISTAN
- Behjat Ul Mudassir: This method has been adapted from Sambrook and Russel, 2006.
Protocol Citation: Behjat Ul Mudassir 2024. EXTRACTION OF DNA BY PHENOL CHLOROFORM METHOD.. protocols.io https://dx.doi.org/10.17504/protocols.io.bp2l62jm5gqe/v1
Manuscript citation:
Cold spring Harb Protoc ; 2006; DOI: 10. 1101/pdb.prot4455
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: October 13, 2024
Last Modified: October 14, 2024
Protocol Integer ID: 109772
Keywords: Phenol-Chloroform DNA Extraction Protocol, Pure DNA Extraction from Human Blood, Blood sample DNA extraction
Abstract
The phenol-chloroform extraction method is often used to isolate and purify DNA from biological specimens. This approach allows the extraction of high-quality DNA appropriate for numerous molecular biology experiments.
Attachments
Extraction of DNA by Phenol-Chloroform Method.
Extraction of DNA by Phenol-Chloroform Method.
Cell Lysis
Denaturation of proteins
Denaturation of proteins and other cellular debris into phases
DNA recovery as pellet
Collection of DNA pellet
Washing, Storage, resuspension of pellets