If using broth, pick single colony and grow in 5 ml Trypticase Soy Broth (TSB), overnight grow at 37 degree without shaking. Use 0.4 ml of the bacterial culture and centrifuge for 4min at 8000 rpm, discard supernatant and resuspend the cell pellet in 100 μL of Resuspension Buffer (R4) containing RNase A, then add 5 ul of lysozyme solution (50 mg/ML). Mix by vortexing. After adding RNase A to Resuspension Buffer (R4), store the buffer at 4 degrees Celsius.