Jul 06, 2022
Expression of molecular markers in subpopulations of mouse stellate ganglion neurons
- 1Columbia University Irving Medical Center
Protocol Citation: Daniele Neri, Lori Zeltser 2022. Expression of molecular markers in subpopulations of mouse stellate ganglion neurons. protocols.io https://dx.doi.org/10.17504/protocols.io.8epv592bdg1b/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it’s working
Created: April 01, 2022
Last Modified: July 06, 2022
Protocol Integer ID: 60205
Abstract
This protocol is a direct application of the original RNAScope Fluroescent Multiplex Reagent Kit (ACDBio, Document Number 320293-USM, https://acdbio.com/sites/default/files/320293_RNAscope%20Multiplex_UM_04102019%20%281%29.pdf)
Materials
| A | B | C | |
| Instrument | Provider | Cat. No/SKU | |
| Slide Moat | Boekel Scientific | 240000 / 240000-2 | |
| 5.5 Quart Digital Steamer | Hamilton Beach | 37530Z | |
| Digital Thermometers with Stainles-Steel Probe on Cable | Fisherbrand | 15-077-9E | |
| Hydrophobic Barrier Pen | Vector Laboratories | NC9545623 | |
| Coplin Jars | FisherScientific | 08-813D | |
| Microscope Slides | FisherScientific | 1255015 | |
| Cover Glasses: Rectangles | FisherScientific | 12544EP |
| A | B | C | |
| Reagent | Provider | Cat.no/SKU | |
| Fluorescent Multiplex Detection Reagents | Advanced Cell Diagnostics | 320851 | |
| RNAse-Free Water | FisherScientific | BP5611 | |
| RNase Decontamination Solution | FisherScientific | AM9780 | |
| Phosphate Buffered Saline | FisherScientific | BP3994 | |
| VECTASHIELD® HardSet™ Antifade Mounting Medium | Vector Laboratories | H-1400-10 | |
| RNAscope® Probe- Mm-Fst | Advanced Cell Diagnostics | 454331 | |
| RNAscope® Probe- Mm-Sctr | Advanced Cell Diagnostics | 508511 | |
| RNAscope® Probe- Mm-Sctr-C2 | Advanced Cell Diagnostics | 508511-C2 | |
| RNAscope® Probe- Mm-Rxfp1 | Advanced Cell Diagnostics | 458001 | |
| RNAscope® Probe- Mm-Rxfp1-C3 | Advanced Cell Diagnostics | 458001-C3 | |
| RNAscope® Probe- Mm-Cckar | Advanced Cell Diagnostics | 313751 | |
| RNAscope® Probe- Mm-Aqp1 | Advanced Cell Diagnostics | 504741 | |
| RNAscope® Probe- Mm-Mpz | Advanced Cell Diagnostics | 573461 | |
| RNAscope® Probe- Mm-Ptk2b | Advanced Cell Diagnostics | 506861 | |
| RNAscope® Probe- Mm-Npy-C2 | Advanced Cell Diagnostics | 313321-C2 | |
| RNAscope® Probe- Mm-Npy-C3 | Advanced Cell Diagnostics | 313321-C3 | |
| RNAscope® Probe- Mm-Colq-C2 | Advanced Cell Diagnostics | 496211-C2 | |
| RNAscope® Probe- Mm-Slc18a1-C3 | Advanced Cell Diagnostics | 542761-C3 | |
| RNAscope® Probe- GFP-C3 | Advanced Cell Diagnostics | 409011-C3 | |
| RNAscope® Probe- Mm-Man1a-C3 | Advanced Cell Diagnostics | 547391-C3 |