A | B | |
Expression system | E.Coli BL21DE3 | |
Medium | Luria Bertani | |
Plasmid origin | ||
Backbone | pGST2 | |
Resistance | Amp | |
Insert | ||
Tags & cleavage sites | N-term GST | |
Ext coeff | 82320 M-1cm-1, MW 77.9 kDa |
A | B | |
Hepes pH=7.5 | 50 mM | |
NaCl | 300 mM | |
TCEP | 1 mM | |
Protease Inhibitors (Roche) |
A | B | |
Hepes pH=7.5 | 50 mM | |
NaCl | 150 mM | |
TCEP | 1 mM |
A | B | |
Hepes pH=7.5 | 50 mM | |
NaCl | 150 mM | |
TCEP | 1 mM | |
Glutathione | 50 mM |
A | B | |
Hepes pH=8 | 20 mM | |
NaCl | 150 mM | |
TCEP | 1 mM |