Pellets were homogenized 20 times by Pyrex douncer (Corning) in lysis buffer (25 mM HEPES pH 7.5, 200 mM NaCl, 2 mM MgCl2, 10% Glycerol) with 25mM TCEP/proteinase inhibitors (Thermo Scientific), and then add 10% Triton X-100 stock to final 1% concentration.