Molecular weight: 80384.47 Da
Ext. coefficient: 77240 M-1*cm-1
Abs 0.1% (=1 g/l) 0.961, assuming all Cys residues are reduced
pETDuet-1_6xHis-TEV-mCh-NDP52 (Addgene ID: 187829)
E. coli Rosetta pLysS cells
LB medium with antibiotics: 50 μl/ml ampicillin and 34 μl/ml chloramphenicol
IPTG (Isopropyl-β-D-thiogalactopyranosid)
Complete inhibitor EDTA free Roche
2 mM Beta-Mercaptoethanol
2 mM Beta-Mercaptoethanol
5-ml His-Trap column (Cytiva)
Superdex 200 increase 16/600 column (Cytiva)