This tutorial introduces Qiime2 for non-phylogenetic diversity
analysis, covering core metrics calculation, emphasizing standardized sampling
depth, and using pre-processed feature tables and metadata. It then explores
beta group significance analysis via qiime diversity beta-group-significance,
detailing parameters and introducing pairwise testing. The tutorial concludes
with a concise demonstration of PCoA plot creation using qiime emperor plot,
highlighting input requirements and insights into result interpretation. Users
are encouraged to explore the interactive Qiime2 visualization for valuable
insights into microbial community relationships and factors influencing
diversity. Overall, the tutorial serves as a brief yet comprehensive
introduction to Qiime2 for enhanced capabilities in microbiome research.