Flies are kept in incubator with cooling @ 18-25 °C, humidity 60-75% with light-dark cycles, 12 hours of dark from 8pm to 8am, and 12 hours of light from 8am to 8pm (Figure 2.). In case that you want to breed flies in the optimum condition of 25°C and 60% humidity generation time is 9-10 days from egg to adult, while in lower temperature such as 18 °C generation time is longer. Higher temperatures increase aging, useful when in experiment one wants to have old flies and compare it to young. In the case that you do not have automatic setting of humidity in the incubator, a container with distilled water can be placed, which will ensure the necessary humidity, which is then controlled from the inside. Same it is with light and dark control. It is possible to buy timer and LED strip, and place it in the incubator in the way that LED strip is attached to the inside of the incubator, while the lighting is controlled via timer connected to socket inside the incubator.