Overdose the mouse with a 1 mLintraperitoneallyinjection of a 150 mg/kg ketamine and 30 mg/kg xylamzine mix. While mouse is overdosing: place the slice chamber in a heated water bath set to 37 °C (water in the bath should come up the side of the chamber to approximately the same height as the aCSF). Next, after mouse is completely anesthetized:
-Transcardially perfuse mouse with the ice-cold 1x aCSF.
-Rapidly decapitate and extract brain in 1x aCSF slush.
-Using razor blade, cut brain down the midline.
-Glue brain medial side down on cutting block and place block quickly into cutting chamber of a Vibratome.
-Fill cutting chamber with aCSF slush solution, kept continuously carbogenated in chamber.
-Cut slices at 240 µmthick
-Quickly transfer each cut slice into hold chamber kept in heated bath.
-Remove holding chamber from bath 30 minutes after last slice transfer is made.
-Allow holding chamber to equilibrate to room temperature (approximately 20 minutes) before transferring slices to recording chamber of electrophysiological rig.