Dec 12, 2023
Establishment of patient-derived enteroids/colonoids from endoscopic biopsies
- 1Children's Hospital of Philadelphia, Gastrointestinal Epithelium Modeling Program
Protocol Citation: Tatiana Karakasheva 2023. Establishment of patient-derived enteroids/colonoids from endoscopic biopsies. protocols.io https://dx.doi.org/10.17504/protocols.io.14egn3rxql5d/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: December 12, 2023
Last Modified: December 12, 2023
Protocol Integer ID: 92203
Funders Acknowledgement:
Children's Hospital of Philadelphia Research Institute
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Abstract
This protocol describes the full procedure of generating an enteroid or colonoid culture from endoscopic biopsy (either freshly procured or recovered from cryopreservation), was developed for human tissue, but works equally well for murine tissue fragments.
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