- Vortex + benchtop centrifuge for 5 mL tubes
- Horizontal vortex with 5 mL tube holder (15 mL tube holders)
- Centrifuge for 2 mL tubes (relative centrifugal force needed: 11,000 x g)
- MagnetaPure 32 Nucleic Acid Purification System (Dutscher)
- Specific DNA/RNA-workstation (sterile area equipped with air filtration and UV systems)
All tubes and tips must be sterile
- 1000 µL tips with filter
- 100 µL tips with filter
- 50 mL tubes: 4 to prepare aliquots, 3 for scissors decontamination 1 for rDNase dilution.
- 5 mL tubes: 1 per 8 samples to prepare NucleoMag B-Beads and MWA2 mix
- 2 mL tubes: 1 per sample to transfer lysate + 2 to prepare aliquots
- 0.5 mL tubes: 3 per sample to transfer eluted RNA
- 96-well plate with 2 mL deep-wells, U-Bottom (Macherey Nagel - 746032.DEEP): 1 per 16 samples
- Magnetic rod coverfor MagnetaPure 32 (Macherey Nagel – 747032.TC): 1 per 8 samples
- Plastic film to protect the 96-well plate
For any manipulation in a rare DNA/RNA room, provide complete equipment (disposable coat, cap, mask, shoe covers & gloves).
- rDNAse Set (Macherey Nagel - 740963)
- Reaction Buffer for rDNase (Macherey Nagel - 740834.60)
- NucleoMag DNA/RNA Water kit (Macherey Nagel)
Note: shelf life of reagents for 24 months from production
o Solution to remove RNA (e.g.RNase-off, RNA away)
o Ethanol 96% for molecular biology
o Molecular biology grade water
- Filters in 5 mL tubes and stored at -80°C until RNA extraction