Mar 21, 2025

Public workspaceER-phagy Experiments

DOI
dx.doi.org/10.17504/protocols.io.6qpvr9qxovmk/v1
  • 1Sascha Martens lab, University of Vienna, Max Perutz Labs - Vienna
Elias Adriaenssens
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DOI: dx.doi.org/10.17504/protocols.io.6qpvr9qxovmk/v1
Protocol Citation: Elias Adriaenssens 2025. ER-phagy Experiments. protocols.io https://dx.doi.org/10.17504/protocols.io.6qpvr9qxovmk/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: January 22, 2025
Last Modified: March 21, 2025
Protocol Integer ID: 119234
Keywords: ASAPCRN
Funders Acknowledgements:
Marie Skłodowska-Curie MSCA Postdoctoral fellowship
Grant ID: 101062916
Aligning Science Across Parkinson’s (ASAP)
Grant ID: ASAP-000350
Abstract
This protocol details about the ER-phagy experiment.
Materials
Reagents:

  • ReagentEarle′s Balanced SaltsMerck MilliporeSigma (Sigma-Aldrich)Catalog #E3024

Procedure
Procedure
12h
12h
Seed HeLa cells in 6-well plates (1 million cells/well)
To induce ER-phagy, wash the cells with PBS first, and then starve the cells for Duration12:00:00 by culturing them in Earle’s balanced salt medium (Cat# E3024, Sigma-Aldrich).

12h
Collect the cells, lyse them in RIPA buffer, and analyze the cells by SDS–PAGE and western blot analysis.