Lyse cells in 1% (v/v) TX-100 in PBS supplemented with protease inhibitors (1 mM PMSF, 1 mg/ml leupeptin, 1 mg/ml pepstatin A). Lysates need to be at least 2 mg/ml. Therefore lyse 6-well in 100 ml. Incubate on ice, 15 minutes. Pellet debris at full speed in microfuge, 10 min, 4 °C.