HEK293 (ATCC CRL-1573, RRID: CVCL_0045 )
anti-YIPF4 (Sino Biological 202844-T46)
anti-GOLGB1/ Giantin (abcam ab37266)
PBS; Phosphate buffered saline: ThermoFisher (#14040133)
PhosSTOP (Sigma-Aldrich, 4906845001)
NuPAGE™ LDS Sample Buffer (4X): Invitrogen (#NP0007)
DTT (dithiothreitol): Thermo-Fisher (#R0861)
Pierce™ Rapid Gold BCA Protein Assay Kit: Thermo Scientific (#A53226)
Immobilon®-P Membrane, PVDF: Milipore (#IPVH85R)
Protease inhibitor cocktail: Sigma-Aldrich (#P8340),
PhosSTOP: Sigma-Aldrich (#4906845001)
Invitrogen, NuPAGE™ MOPS SDS Running Buffer (#NP0001)
TBST, TRIS-buffered saline (TBS, 20X), with 2% Tween 20 (#J60497.K3)
Ponceau S, Electrophoresis Grade, Thermo Scientific™ (#J60744.18)
Benzonase Nuclease HC: Millipore (#71205-3)
Dulbecco’s MEM (DMEM), high glucose, pyruvate (Gibco / Invitrogen, 11995)
AlexaFluor 594 Goat anti-Rabbit IgG, ThermoFisher (#A-11012)
Vectashield H-1000: Cole-Parmer (#3304766)
repair template containing an mNEON Green cassette flanked by homology arms (pSMART-mNEON-YIPF4): Insert sequence: