Dec 01, 2023
- 1Massachusetts General Hospital, ASAP
Protocol Citation: Pranay Srivastava 2023. ELISA. protocols.io https://dx.doi.org/10.17504/protocols.io.kqdg3xw67g25/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: November 30, 2023
Last Modified: May 31, 2024
Protocol Integer ID: 91668
Keywords: ASAPCRN
Abstract
This protocol is to determine the levels of IL-1β and TNF-α in plasma and brain (Srivastava et al. 2020).
Blood samples from all the treatment groups were collected through cardiac puncture in 40 mM EDTA and plasma was collected by centrifugation.
The plasma samples were immediately transferred to dry ice and then stored at -80°C till further analysis.
A small fraction of ventral midbrain tissue homogenate prepared in 1x RIPA buffer (Cell Signaling, Cat#9806) was used for analysis of IL-1β and TNF-α using mouse ELISA kits (Biolegend Cat# 430904; 432604).