PI3KC3-C1(VPS15-TSF) and PI3KC3-C1(mCherry-ATG14|VPS15-TSF) protein samples
5 mL PD-10 column (Cytiva)
0.5 M NH4CH3CO2 buffer
Nanodrop spectrophotometer
Liquid chromatography (LC) system (Agilent 1200 series)
LTQ-Orbitrap-XL mass spectrometer with ESI source (Thermo Fisher Scientific)
Ammonium acetate (≥98% purity)
Methanol (Optima LC-MS grade, ≥99.9% purity)
Purified water (resistivity of 18.2 MΩ·cm)
Ultra C18 column (length: 150 mm, inner diameter: 2.1 mm, particle size: 3 µm)
Pierce LTQ ESI positive ion calibration solution (Thermo Fisher Scientific)
Xcalibur software (version 2.0.7, Thermo Fisher Scientific)